CRYOSCANNING ELECTRON-MICROSCOPY OF A LAYER OF EXTRACELLULAR POLYSACCHARIDES PRODUCED BY BACTERIAL COLONIES

被引：15

作者：

VANDOORN, WG ^{[1
]}

THIEL, F ^{[1
]}

BOEKESTEIN, A ^{[1
]}

机构：

[1] TECH & PHYS ENGN RES SERV,WAGENINGEN,NETHERLANDS

来源：

SCANNING | 1990年 / 12卷 / 06期

关键词：

D O I：

10.1002/sca.4950120603

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Cryo‐SEM allowed observation of a layer of extracellular polysaccharides that covered colonies of the bacterium Pseudomonas aeruginosa, growing on agar substrate. The layer was not found when using conventional preparation techniques and scanning electron microscopy. It disappeared partially as a result of glutaraldehyde fixation, but mainly during alcohol dehydration and critical point drying. Copyright © 1990 Foundation for Advances in Medicine and Science, Inc.

引用

页码：297 / 299

页数：3

共 12 条

[1]

DEWITTE Y, 1988, SCI HORT, V32, P285

[2] COMPOSITION OF AN EXTRACELLULAR SLIME PRODUCED BY PSEUDOMONAS AERUGINOSA [J].