WARM CAROLINA RINSE SOLUTION PREVENTS GRAFT FAILURE FROM STORAGE INJURY AFTER ORTHOTOPIC RAT-LIVER TRANSPLANTATION WITH ARTERIALIZATION

被引：15

作者：

BACHMANN, S ^{[1
]}

CALDWELLKENKEL, JC ^{[1
]}

OLEKSY, I ^{[1
]}

STEFFEN, R ^{[1
]}

THURMAN, RG ^{[1
]}

LEMASTERS, JJ ^{[1
]}

机构：

[1] UNIV N CAROLINA,SCH MED,DEPT CELL BIOL & ANAT,CELL BIOL LABS,CAMPUS BOX 7090,236 TAYLOR HALL,CHAPEL HILL,NC 27599

来源：

TRANSPLANT INTERNATIONAL | 1992年 / 5卷 / 02期

关键词：

RAT LIVER TRANSPLANTATION; CAROLINA RINSE SOLUTION; LIVER TRANSPLANTATION; RAT; PRESERVATION; LIVER;

D O I：

10.1007/BF00339225

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

An injury to nonparenchymal cells, characterized by loss of viability of sinusoidal endothelial cells and activation of Kupffer cells, occurs after reperfusion of livers stored for transplantation. Recently, a new solution, Carolina rinse solution, was shown to prevent reperfusion injury to endothelial cells in vitro almost completely and to improve graft survival after orthotopic rat liver transplantation (ORLT) without arterialization. ORLT with arterialization permits longer cold storage of donor livers and more closely models human surgery. Therefore, we evaluated the effects of Carolina rinse solution on graft survival after ORLT with arterialization in syngeneic Lewis rats. Just prior to implantation, donor livers stored in University of Wisconsin (UW) solution were rinsed with 30 ml of Ringer's solution, saline, or Carolina rinse solution at l-degrees-4-degrees-C. In livers stored for 15 h and rinsed with Ringer's or saline solution. long-term graft survival was only 8%. Using Carolina rinse solution containing 1 mmol and 200-mu-mol adenosine per liter, graft survival improved to 40% and 80%, respectively. Graft survival did not improve when using Carolina rinse solution with adenosine omitted or Ringer's solution containing 200-mu-mol adenosine per liter. Livers were also rinsed with Carolina rinse solution containing 200-mu-mol adenosine per liter at 28-degrees-30-degrees-C rather than at 1-degrees-4-degrees-C. With warm Carolina rinse solution, survival improved further to 100%, 80%, and 50% after 15, 18, and 21 h of storage. After 18 h of storage, light and electron microscopy demonstrated marked denudation of the sinusoidal lining and activation of Kupffer cells in grafts rinsed with Ringer's solution. Use of Carolina rinse solution greatly improved endothelial structure but did not reduce Kupffer cell activation. In conclusion, these findings show that Carolina rinse solution substantially improves graft survival after ORLT with arterialization. Adenosine and warm temperature are important factors contributing to efficacy. A mechanism of protection appears to be prevention of reperfusion-induced endothelial cell injury.

引用

页码：108 / 114

页数：7

共 36 条

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