MEASUREMENT OF INTRACELLULAR PH AND ASPECTS OF ITS CONTROL IN HIGHER PLANT-CELLS CULTIVATED IN LIQUID-MEDIUM

被引：43

作者：

KURKDJIAN, A

LEGUAY, JJ

GUERN, J

机构：

[1] Laboratoire de Génétique et Physiologie du Développement des Plantes, Service de Physiologie Cellulaire, CNRS

来源：

RESPIRATION PHYSIOLOGY | 1978年 / 33卷 / 01期

关键词：

4-D Auxin Intracellular pH Cell division; Acer pseudoplatanus CO[!sub]2[!/sub] Ammonium ions 2;

D O I：

10.1016/0034-5687(78)90086-5

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The 5,5-dimethyloxazolidine-2,4-dione (DMO) method for the determination of intracellular pH has been adapted to Acer pseudoplatanus cells cultivated in liquid medium. DMO is metabolized by these cells and this parameter has to be taken into account in the measurement of the intracellular concentration of free DMO. As the cells contain a large acidic vacuolar compartment, the intracellular pH estimated from the DMO technique is an 'overall pHi', the dependence of which on various cytoplasmic and vacuolar characteristics has been discussed. Important modifications of these overall pHi values under the influence of CO2 or NH+4 have been measured. These pHi modifications strongly affect the regulation of cell division through the control of the intracellular concentration of hormones of the auxin type. © 1978.

引用

页码：75 / 89

页数：15

共 20 条

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