CELLULAR IMMUNE-RESPONSE IN RAINBOW-TROUT SALMO-GAIRDNERI RICHARDSON TO YERSINIA-RUCKERI O-ANTIGEN MONITORED BY THE PASSIVE HEMOLYTIC PLAQUE-ASSAY TEST

被引：31

作者：

ANDERSON, DP ^{[1
]}

ROBERTSON, BS ^{[1
]}

DIXON, OW ^{[1
]}

机构：

[1] UNIV MARYLAND,COLLEGE PK,MD 20742

来源：

JOURNAL OF FISH DISEASES | 1979年 / 2卷 / 03期

关键词：

D O I：

10.1111/j.1365-2761.1979.tb00156.x

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Abstract. The specificity and kinetics of the immune response of rainbow trout (Salmo gairdneri) to single injections of an O‐antigen extracted from the bacterial pathogen Yersinia ruckeri, which causes enteric redmouth in fish, were investigated by the passive haemolytic plaque assay and serum antibody quantitation. Doses ranging from 5 ng to 500 mg in 10‐fold increments were injected intraperitoneally into groups of trout held at 17 × 1°5°C. The occurrence of plaque forming cells (PFC) and humoral antibody was followed for 35 days after injection. Trout gave an immune response to doses of 500 ng and above. Seven days after injection no humoral antibody was detected, but PFC were found in the spleen. The maximum PFC numbers occurred 11 days after injection. On day 21, few PFC were found, whereas serum antibody titres were highest. The antibody from immunized trout showed little or no cross‐reactions with sheep red blood cells passively labelled With antigens from other fish pathogens. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：169 / 178

页数：10

共 16 条

[1]

Avtalion R R, 1973, Curr Top Microbiol Immunol, V61, P1

[2]

AVTALION RR, 1977, ISRAEL J MEDICAL SCI, V11, P1385

[3] USE OF ERYTHROCYTES SENSITIZED WITH PURIFIED PNEUMOCOCCAL POLYSACCHARIDES FOR ASSAY OF ANTIBODY AND ANTIBODY-PRODUCING CELLS [J].