ANTIOXIDANT ENZYMES AND LIPOPEROXIDE IN BLOOD IN UREMIC CHILDREN AND ADOLESCENTS

被引：43

作者：

ASAYAMA, K

SHIKI, Y

ITO, H

HASEGAWA, O

MIYAO, A

HAYASHIBE, H

DOBASHI, K

KATO, K

机构：

[1] Department of Pediatrics, Yamanashi Medical College, Tamahocho

[2] Department of Pediatrics, School of Medicine, Keio University, Tokyo

[3] Department of Pediatric Nephrology, Tokyo Metropolitan Children's Hospital, Tokyo

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1990年 / 9卷 / 02期

关键词：

Chronic renal failure; Free radicals; Hemodialysis; Hyperlidemia; Leukocytes; Lipid peroxide; Radioimmunoassay; Superoxide dismutase;

D O I：

10.1016/0891-5849(90)90112-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To determine whether oxidant-antioxidant balance is altered in chronic renal failure, antioxidant enzymes and lipid peroxide in peripheral blood cells and lipid peroxide in plasma were measured. Nine children and adolescents maintained on hemodialysis (HD), 9 on continuous ambulatory peritoneal dialysis (CAPD), and 14 controls were studied. Lipid peroxide was assayed fluorimetrically as thiobarbituric acid-reactive substance, superoxide dismutases by radioimmunoassays. Both manganese and copper-zinc superoxide dismutases in lymphocytes and monocytes in the HD and CAPD patients, and manganese superoxide dismutase in polymorphs in the HD patients were higher than in the controls. Copper-zinc superoxide dismutase, glutathione peroxidase, and catalase in erythrocytes were unaltered. The lipid peroxide level in plasma in the dialyzed patients was increased, whereas those in polymorphs and lymphocytes were unaltered. Triglyceride and total cholesterol in plasma in the dialyzed patients were also increased. The plasma lipid peroxide in the patients correlated with the triglyceride and total cholesterol level. This is the first study in which manganese superoxide dismutase is measured in nucleated cells of the patients with chronic renal failure. The present results suggest that increased superoxide dismutases protect against oxidative stress induced by chronic renal failure in nucleated cells but in either erythrocytes nor plasma. © 1990.

引用

页码：105 / 109

页数：5

共 30 条

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