RECOMBINANT AEQUORIN AS A PROBE FOR CYTOSOLIC FREE CA2+ IN ESCHERICHIA-COLI

被引：76

作者：

KNIGHT, MR ^{[1
]}

CAMPBELL, AK ^{[1
]}

SMITH, SM ^{[1
]}

TREWAVAS, AJ ^{[1
]}

机构：

[1] UNIV COLL CARDIFF,DEPT MED BIOCHEM,CARDIFF CF4 4XN,S GLAM,WALES

来源：

FEBS LETTERS | 1991年 / 282卷 / 02期

关键词：

AEQUORIN RECONSTITUTION; COELENTERAZINE; ESCHERICHIA-COLI; COMPLEMENT; PHAGOCYTOSIS; ANTIBIOTIC; CYTOPLASMIC-CA2+;

D O I：

10.1016/0014-5793(91)80524-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a novel and simple method for the measurement of bacterial cytosolic free calcium ([Ca2+]i) using recombinant aequorin reconstituted within live bacterial cells. Using this method we have measured the effects of external calcium, complement, phagocytosis and antibiotics on the [Ca2+]i of Escherichia coli. In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems [Ca2+]i of Escherichia coli. In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems than fluorescent dyes for subcellular calcium measurement.

引用

页码：405 / 408

页数：4

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