CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDY OF GLUCOSE-DEHYDROGENASE FROM THE ARCHAEBACTERIUM THERMOPLASMA-ACIDOPHILUM

被引：4

作者：

BRIGHT, JR

MACKNESS, R

DANSON, MJ

HOUGH, DW

TAYLOR, GL

TOWNER, P

BYROM, D

机构：

[1] UNIV BATH,DEPT BIOCHEM,CLAVERTON DOWN,BATH BA2 7AY,AVON,ENGLAND

[2] ICI BIOL PROD,BILLINGHAM TS23 1LB,CLEVELAND,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 222卷 / 02期

关键词：

ARCHAEBACTERIA; GLUCOSE DEHYDROGENASE; CRYSTALLOGRAPHY;

D O I：

10.1016/0022-2836(91)90200-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single crystals of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum were obtained using the hanging-drop vapour diffusion method and polyethylene glycol as a precipitant in the presence of NADP+ at pH 5.4. The crystals belong to the hexagonal space group P6122 or P6522, with unit cell dimensions a = b = 121.9 A ̊, c = 229.6 A ̊ and with two molecules in the asymmetric unit. © 1991.

引用

页码：143 / 144

页数：2

共 7 条

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[6] CRYSTALLIZATION OF AND X-RAY-INVESTIGATIONS ON GLUCOSE-DEHYDROGENASE FROM BACILLUS-MEGATERIUM
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[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 167 (01): : 123 - 124
[7] PURIFICATION AND CHARACTERIZATION OF GLUCOSE-DEHYDROGENASE FROM THE THERMOACIDOPHILIC ARCHAEBACTERIUM THERMOPLASMA-ACIDOPHILUM
SMITH, LD
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[J]. BIOCHEMICAL JOURNAL, 1989, 261 (03) : 973 - 977

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