FAST DEVELOPMENT OF A ROBUST HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR GINKGO-BILOBA BASED ON COMPUTER-SIMULATION

Rapid development of robust and reliable high-performance liquid chromatographic methods for routine quality control of Ginkgo biloba is possible with computer simulation. The goal is to reduce method development time and to increase transparency of the complex composition of plant extracts. With only two basic experiments and a peak tracking process based on the total area ratio compared to the individual peak-area ratios a robust method with more than 50 simulated experiments was completed in 8 h. The best method has been verified experimentally. The correlation between the best simulated run and the final experiment was satisfactory.