BACULOVIRUS-EXPRESSED GLYCOPROTEIN-E (GE) OF HERPES-SIMPLEX VIRUS TYPE-1 (HSV-1) PROTECTS MICE AGAINST LETHAL INTRAPERITONEAL AND LETHAL OCULAR HSV-1 CHALLENGE

被引:40
作者
GHIASI, H [1 ]
KAIWAR, R [1 ]
NESBURN, AB [1 ]
SLANINA, S [1 ]
WECHSLER, SL [1 ]
机构
[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT OPHTHALMOL,LOS ANGELES,CA 90024
关键词
D O I
10.1016/0042-6822(92)90500-O
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have constructed a recombinant baculovirus expressing high levels of the herpes simplex virus type 1(HSV-1) glycoprotein E (gE) in Sf9 cells. The expressed gE migrated on gels as a double band with apparent molecular weights of 68 and 70 kDa. The recombinant gE was glycosylated based on its susceptibility to tunicamycin treatment and was transported to the membrane of Sf9 cells based on indirect immunofluorescence. Mice vaccinated with gE developed high serum titers of HSV-1-neutralizing antibodies based on plaque reduction assays. gE vaccination also induced a strong delayed type hypersensitivity (DTH) response to HSV-1. In addition, mice vaccinated with the recombinant gE were protected from both intraperitoneal and ocular lethal HSV-1 challenge. To our knowledge, this is the first report in which vaccination with gE was shown to induce high neutralizing antibody titers, a DTH response, or protection against lethal HSV-1 challenge. © 1992.
引用
收藏
页码:469 / 476
页数:8
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