GANGLIO-N-TETRAOSYLCERAMIDE (GA1) OF BOVINE AND HUMAN BRAIN - MOLECULAR CHARACTERIZATION AND PRESENCE IN MYELIN

被引：20

作者：

DASGUPTA, S ^{[1
]}

VANHALBEEK, H ^{[1
]}

HOGAN, EL ^{[1
]}

机构：

[1] UNIV GEORGIA,COMPLEX CARBOHYDRATE RES CTR,ATHENS,GA 30602

来源：

FEBS LETTERS | 1992年 / 301卷 / 02期

关键词：

BOVINE BRAIN; MYELIN; NEUTRAL GLYCOSPHINGOLIPIDS; NMR;

D O I：

10.1016/0014-5793(92)81234-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During our studies of bovine brain neutral glycosphingolipids (Ngsl's), we have purified a compound that co-migrates on thin-layer chromatogram with standard GA1 (purified by acid hydrolysis of GM1) and close to penta-(nLc5Cer) glycosylcermide from bovine erthrocytes. The structure of the purified Ngsl from brain has been established by permethylation and by stepwise exoglycosidase hydrolysis. 600 MHz H-1 NMR spectroscopy of the oligosaccharide obtained from the Ngsl after endoglycoceramidase hydrolysis confirms the structure as ganglio-N-tetraosylceramide (GgOse4Cer or Ga1) as Ga1-beta-1 --> 3Ga1NAc-beta-1 --> 4Ga1-beta-1 --> 4G1c-beta-1 --> Cer. We have identified GA1 in bovine, rat and human brain and myelin by TLC-immunostaining with monospecific anti-GA1 antiserum.

引用

页码：141 / 144

页数：4

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