A STUDY OF COLCHICINE TUBULIN COMPLEX BY DONOR QUENCHING OF FLUORESCENCE ENERGY-TRANSFER

被引：10

作者：

BHATTACHARYYA, A

BHATTACHARYYA, B

ROY, S

机构：

[1] BOSE INST,DEPT BIOPHYS,P 1-12,CIT SCHEME VII M,CALCUTTA 700054,INDIA

[2] BOSE INST,DEPT BIOCHEM,CALCUTTA 700009,W BENGAL,INDIA

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 216卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1993.tb18195.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The utility of collisional quenching of energy donors in fluorescence energy transfer is described. In multi-donor single acceptor systems, which contain different classes of donors (as distinguished by their accessibility towards a collisional quencher), donor quenching may be used to assess the fraction of energy transfer from each class of donor. The tubulin-colchicine complex was used as a donor-acceptor system to show that two inaccessible tryptophans are at or near the colchicine binding site.

引用

页码：757 / 761

页数：5

共 26 条

[1] FLUORESCENCE QUENCHING AND ENERGY-TRANSFER IN COMPLEXES BETWEEN HORSE-LIVER ALCOHOL-DEHYDROGENASE AND COENZYMES [J].