AN ALTERNATIVE ISOLATION PROCEDURE FOR THE SUBSEQUENT DETERMINATION OF BENZO(A)PYRENE IN TOTAL PARTICULATE MATTER OF CIGARETTE-SMOKE

A solid phase extraction procedure (Sep-Pak cartridge; Waters; Milford, MA) with an amino stationary phase was used for the isolation of benzo(a)pyrene (B[a]P) in cigarette smoke condensate before analysis by high-performance liquid chromatography. Compared with previously reported isolation techniques, the disposable Sep-Pak cartridge provides a much faster isolation and allows many samples to be prepared simultaneously for HPLC analysis. Recovery of B(a)P was high, 94±2%, and B(a)P deliveries from 1R1 and 1R4F Kentucky reference cigarettes were in good agreement with previously reported values. © 1994, Oxford University Press.