IMPROVED METHOD FOR RAPID ASSAYING OF VIABILITY OF CRYOPRESERVED UNICELLULAR ALGAE

被引：12

作者：

GILBOA, A

BENAMOTZ, A

机构：

[1] Israel Oceanographic and Limnological Research Ltd., Haifa

来源：

PLANT SCIENCE LETTERS | 1979年 / 14卷 / 04期

关键词：

D O I：

10.1016/S0304-4211(79)90245-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A method of assaying the viability of cryopreserved unicellular algae through photosynthetic oxygen evolution of cryopreserved cells permits a rapid estimation of viability with regard to the usual plating technique. Comparative studies between the two methods show that estimation of viability photosynthetically is rapid and accurate. The method, which was tested with three marine unicellular algae; Chlorella stigmatophora, Platymonas suecica and Phaeodactylum tricornutum, showed successful estimation of viability after a two-step cooling technique in the presence of the cryoprotectant dimethylsulfoxide. © 1979 Elsevier/North-Holland Scientific Publishers Ltd.

引用

页码：317 / 320

页数：4

共 16 条

[1] COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].