ISOLATION AND RNA SEQUENCE-ANALYSIS OF CYTOCHROME-B MUTANTS RESISTANT TO FUNICULOSIN, A CENTER I-INHIBITOR OF THE MITOCHONDRIAL UBIQUINOL-CYTOCHROME-C REDUCTASE IN SACCHAROMYCES-CEREVISIAE

被引：35

作者：

DIRAGO, JP

PEREA, J

COLSON, AM

机构：

[1] CATHOLIC UNIV LOUVAIN, GENET MICROBIENNE LAB, B-1348 LOUVAIN LA NEUVE, BELGIUM

[2] CNRS, CTR GENET MOLEC, F-91190 GIF SUR YVETTE, FRANCE

来源：

FEBS LETTERS | 1990年 / 263卷 / 01期

关键词：

(Saccharomyces cerevisiae); Funiculosin resistance; Membrane protein folding; Mitochondrial cytochrome b; Mutational analysis; RNA sequencing;

D O I：

10.1016/0014-5793(90)80713-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Funiculosin is a well-known inhibitor of the mitochondrial respiratory chain, probably acting at the ubiquinone reducing site or center i of QH2 cytochrome c reductase. We report here the isolation, mapping and RNA sequence analysis of yeast apo-cytochrome b mutants resistant to this inhibitor. Funiculosin-resistance was found to be conferred, in 4 independent isolates, upon replacement of a leucine residue by phenylalanine in position 198 of the cytochrome b polypeptide chain. © 1990.

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页码：93 / 98

页数：6

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