CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF PIG-KIDNEY DOPA DECARBOXYLASE

被引：8

作者：

MALASHKEVICH, VN

FILIPPONI, P

SAUDER, U

DOMINICI, P

JANSONIUS, JN

VOLTATTORNI, CB

机构：

[1] UNIV BASEL, BIOCTR, DEPT STRUCT BIOL, KLINGELBERGSTR 70, CH-4056 BASEL, SWITZERLAND

[2] UNIV PERUGIA, DEPT EXPTL MED & BIOCHEM SCI, I-06100 PERUGIA, ITALY

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 224卷 / 04期

关键词：

DOPA DECARBOXYLASE; CRYSTALLIZATION; X-RAY DIFFRACTION; NON-CRYSTALLOGRAPHIC SYMMETRY; PYRIDOXAL-5'-PHOSPHATE;

D O I：

10.1016/0022-2836(92)90477-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DOPA decarboxylase from pig kidney, an α2 dimeric enzyme of Mr = 107,000, has been crystallized by the vapour diffusion method with ammonium sulphate as precipitant. The crystals belong to the space group P62 (or its enantiomer P64) and have unit cell dimensions of a = b = 155·9 A ̊, c = 87·7 A ̊, α = β = 90 °, γ = 120 °. They diffract to 2·6 Å resolution. There is one dimeric molecule per asymmetric unit. Rotation function studies have r000evealed the orientation of the non-crystallographic 2-fold axis of the dimer in the asymmetric unit. © 1992.

引用

页码：1167 / 1170

页数：4

共 28 条

[1]

ALBERT VR, 1987, J BIOL CHEM, V262, P9404

[2]

Arnone A, 1985, TRANSAMINASES, P138

[3]

BORISOV VV, 1985, TRANSAMINASES, P155

[4]

DOMINICI P, 1985, J BIOL CHEM, V260, P583

[5]

DOMINICI P, 1991, INT UNION B, V199, P83

[6] AFFINITY LABELING OF PIG-KIDNEY 3,4-DIHYDROXYPHENYLALANINE (DOPA) DECARBOXYLASE WITH N-(BROMOACETYL)PYRIDOXAMINE 5'-PHOSPHATE - MODIFICATION OF AN ACTIVE-SITE CYSTEINE [J].

DOMINICI, P ;

MARAS, B ;

MEI, G ;

VOLTATTORNI, CB .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 201 (02) :393-397

[7] 3-DIMENSIONAL STRUCTURE OF A PYRIDOXAL-PHOSPHATE-DEPENDENT ENZYME, MITOCHONDRIAL ASPARTATE-AMINOTRANSFERASE [J].

FORD, GC ;

EICHELE, G ;

JANSONIUS, JN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (05) :2559-2563

[8]

GALLAGHER T, 1989, J BIOL CHEM, V264, P12737

[9] EVIDENCE FOR PQQ AS COFACTOR IN 3,4-DIHYDROXYPHENYLALANINE (DOPA) DECARBOXYLASE OF PIG-KIDNEY [J].

GROEN, BW ;

VANDERMEER, RA ;

DUINE, JA .

FEBS LETTERS, 1988, 237 (1-2) :98-102

[10]

HACKERT ML, 1991, INT UNION B, V199, P425

← 1 2 3 →