CHARACTERISTICS OF A NEW L-FUCOSE AND N-ACETYL-D-GALACTOSAMINE SPECIFIC LECTIN FROM THE INTEGUMENT OF GALLERIA-MELLONELLA LARVAE

被引：4

作者：

GRUBHOFFER, L ^{[1
]}

MATHA, V ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI, INST ENTOMOL, CS-37005 CESKE BUDEJOVICE, CZECHOSLOVAKIA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1991年 / 100卷 / 01期

关键词：

D O I：

10.1016/0305-0491(91)90087-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. An agglutinin with affinity for L-fucose and N-acetyl-D-galactosamine was isolated from the integument of Galleria mellonella larvae. 2. The agglutinin was purified by affinity chromatography on CNBr-activated Sepharose 4B coupled by bovine submaxilar mucine. 3. SDS-electrophoresis showed one protein band with a mol. wt of 70 k under nonreducing conditions. 4. The agglutinin appears to be naturally occurring. Neither injury, nor immunization with nonpathogenic bacteria increased its titre in integument homogenates. 5. The possible functions of this lectin in host defence reactions are discussed.

引用

页码：73 / 76

页数：4

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