TEMPERATURE-DEPENDENT CHANGE OF SEROLOGICAL SPECIFICITY OF CANDIDA-ALBICANS NIH-A-207 CELLS CULTURED IN YEAST EXTRACT-ADDED SABOURAUD LIQUID-MEDIUM - DISAPPEARANCE OF SURFACE ANTIGENIC FACTOR-4, FACTOR-5, AND FACTOR-6 AT HIGH-TEMPERATURE

The cells of Candida albicans NIH A-207 strain (A-strain) cultivated in YSLM at high temperatures (37 and 40 degrees C) did not undergo agglutination with the factor sera 4, 5, and 6 in a commercially available factor serum kit, 'Candida Check', and formed a grape-like shape. The mannans isolated from the cells had lost their reactivity against the factor sera in ELISA. It was also revealed by H-1 NMR analysis that the mannans contained neither a phosphate group nor a beta-1,2-linked mannopyranose unit, although these mannans increased the non-reducing terminal alpha-1,3-linked mannopyranose unit. The cells and the mannans prepared by cultivation at such high temperatures followed by 27 degrees C in the same medium entirely recovered the reactivity with the factor sera.