CELLULASE-CATALYZED GLYCOSYLATION REACTIONS - SIMPLE ROUTE TOWARDS A HIGHLY SELECTIVE SYNTHESIS OF OLIGOSACCHARIDES

被引：36

作者：

KARTHAUS, O ^{[1
]}

SHODA, S ^{[1
]}

TAKANO, H ^{[1
]}

OBATA, K ^{[1
]}

KOBAYASHI, S ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC ENGN,DEPT MOLEC CHEM & ENGN,SENDAI,MIYAGI 980,JAPAN

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 | 1994年 / 13期

关键词：

D O I：

10.1039/p19940001851

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Cellulase (EC 3.2.1.4) can be used for the enzyme-catalysed synthesis of oligosaccharides in a mixed buffer/organic solvent system. Besides glucopyranosides and cellooligosaccharides, non-glucosidic saccharides and modified glucoses as glycosyl accepters also react with beta-lactosyl fluoride as glycosyl donor. The reaction proceeds in a completely stereo- and regio-specific manner, giving rise to coupling products having beta (1-->4) glycosidic bonds exclusively. Based on these results, a model for the active site of cellulase (Trichoderma viride) and the orientation of the donor and acceptor therein is proposed.

引用

页码：1851 / 1857

页数：7

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