IMPROVED METHOD FOR ANALYSIS OF GLYCATED HEMOGLOBIN BY ION-EXCHANGE CHROMATOGRAPHY

被引：64

作者：

ECKERBOM, S ^{[1
]}

BERGQVIST, Y ^{[1
]}

JEPPSSON, JO ^{[1
]}

机构：

[1] MALMO GEN HOSP,DEPT CLIN CHEM,S-21401 MALMO,SWEDEN

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1994年 / 31卷

关键词：

DIABETIC CONTROL; METHOD COMPARISON; INTEGRATION; GLYCATED PROTEINS;

D O I：

10.1177/000456329403100409

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The Pharmacia Mono S column for glycated haemoglobin (HbA1c) was compared with Mono S HR 5/5 and three other cation exchange columns to optimize analysis of HbA1c. This column allows a significant decrease in separation time, resulting in a sample throughput of 12 samples per hour. The choice of parameters for integration of peak areas in the chromatograms is very important for reproducible results. Since pure calibrators of HbA1c are not available, the relative contribution of HbA1c to the total amount of haemoglobin is calculated. We have compared valley-to-valley integration with baseline integration to assess reproducibility. Valley-to-valley integration significantly enhances the reproducibility of the method and is therefore preferable for routine analyses of HbA1c.

引用

页码：355 / 360

页数：6