INVITRO STUDIES IN REPRODUCTIVE IMMUNOLOGY .1. SUPPRESSION OF CELL-MEDIATED IMMUNE-RESPONSE BY HUMAN SPERMATOZOA AND FRACTIONS ISOLATED FROM HUMAN SEMINAL PLASMA

被引：100

作者：

MARCUS, ZH ^{[1
]}

FREISHEIM, JH ^{[1
]}

HOUK, JL ^{[1
]}

HERMAN, JH ^{[1
]}

HESS, EV ^{[1
]}

机构：

[1] UNIV CINCINNATI,MED CTR,DEPT BIOL CHEM,CINCINNATI,OH 45267

来源：

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1978年 / 9卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0090-1229(78)90103-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Components of the male mammalian genital tract are capable of inhibiting in vitro cell-mediated immune responses. In the current study, intact human spermatozoa in a concentration of 107 cells/ml inhibited both spontaneous and PHA-M- and Con A-induced blast transformation of normal human peripheral blood lymphocytes. Similar inhibitory properties were found in human seminal plasma using concentrations of 100 and 400 μg/ml. Sephadex G-100 chromatography of seminal plasma yielded five fractions (F1 - F5) which were qualitatively assessed by SDS-polyacrylamide disc gel electrophoresis. Fractions 1 and 4 inhibited spontaneous blast transformation while fractions 1 and 3 inhibited mitogen-induced blastogenesis. Normal leukocyte migration was not affected by fractions. It is concluded that suppression of blast transformation might be due to a spermatozoal or seminal plasma component(s) able to bind receptors on lymphocytes specific for PHA-M and Con-A. © 1978.

引用

页码：318 / 326

页数：9

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