MACROSTRUCTURE OF THE TOMATO TELOMERES

被引:142
作者
GANAL, MW
LAPITAN, NLV
TANKSLEY, SD
机构
[1] Dept. of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, Ithaca
[2] Department of Agronomy, Colorado State University, Fort Collins
关键词
D O I
10.1105/tpc.3.1.87
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The macrostructure of the tomato telomeres has been investigated by in situ hybridization, genomic sequencing, and pulsed-field gel electrophoresis. In situ hybridizations with a cloned telomeric sequence from Arabidopsis thaliana indicated that the telomeric repeat of tomato cross-hybridizes with that of Arabidopsis and is located at all telomeres. Bal31 digestion kinetics confirmed that the tomato telomeric repeat represents the outermost DNA sequence of each tomato chromosome. Genomic sequencing of enriched tomato telomeric sequences, using primers derived from the Arabidopsis sequence, revealed that the consensus sequence of the tomato telomeric repeat is TT(T/A)AGGG compared with the Arabidopsis consensus sequence of TTTAGGG. Furthermore, as shown by pulsed-field gel electrophoresis, the telomeric repeat of tomato is separated by not more than a few hundred kilobases from a previously described 162-base pair satellite DNA repeat of tomato (TGR I) at 20 of the 24 telomeres. Together, these sequences are found in the heterochromatic terminal knob observed in pachytene chromosomes. Therefore, these two repeats determine the structure of 20 of the 24 tomato chromosome ends over approximately 2% of the total chromosome length.
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收藏
页码:87 / 94
页数:8
相关论文
共 35 条
  • [1] HUMAN TELOMERES CONTAIN AT LEAST 3 TYPES OF G-RICH REPEAT DISTRIBUTED NON-RANDOMLY
    ALLSHIRE, RC
    DEMPSTER, M
    HASTIE, ND
    [J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (12) : 4611 - 4627
  • [2] CONSTRUCTION OF YEAST ARTIFICIAL CHROMOSOME LIBRARIES WITH LARGE INSERTS USING FRACTIONATION BY PULSED-FIELD GEL-ELECTROPHORESIS
    ANAND, R
    VILLASANTE, A
    TYLERSMITH, C
    [J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (09) : 3425 - 3433
  • [3] MOLECULAR DESCRIPTION OF TELOMERIC HETEROCHROMATIN IN SECALE SPECIES
    BEDBROOK, JR
    JONES, J
    ODELL, M
    THOMPSON, RD
    FLAVELL, RB
    [J]. CELL, 1980, 19 (02) : 545 - 560
  • [4] GROWTH OF CHROMOSOME ENDS IN MULTIPLYING TRYPANOSOMES
    BERNARDS, A
    MICHELS, PAM
    LINCKE, CR
    BORST, P
    [J]. NATURE, 1983, 303 (5918) : 592 - 597
  • [5] Bernatzky R., 1986, PLANT MOL BIOL REP, V4, P37, DOI [10.1007/BF02672487, DOI 10.1007/BF02672487]
  • [6] IDENTIFICATION OF A TELOMERIC DNA-SEQUENCE IN TRYPANOSOMA-BRUCEI
    BLACKBURN, EH
    CHALLONER, PB
    [J]. CELL, 1984, 36 (02) : 447 - 457
  • [7] TELOMERES - DO THE ENDS JUSTIFY THE MEANS
    BLACKBURN, EH
    [J]. CELL, 1984, 37 (01) : 7 - 8
  • [8] ELECTROPHORETIC SEPARATIONS OF LARGE DNA-MOLECULES BY PERIODIC INVERSION OF THE ELECTRIC-FIELD
    CARLE, GF
    FRANK, M
    OLSON, MV
    [J]. SCIENCE, 1986, 232 (4746) : 65 - 68
  • [9] SEPARATION OF LARGE DNA-MOLECULES BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS
    CHU, G
    VOLLRATH, D
    DAVIS, RW
    [J]. SCIENCE, 1986, 234 (4783) : 1582 - 1585
  • [10] HOMOLOGOUS RECOMBINATION WITHIN SUBTELOMERIC REPEAT SEQUENCES GENERATES CHROMOSOME SIZE POLYMORPHISMS IN P-FALCIPARUM
    CORCORAN, LM
    THOMPSON, JK
    WALLIKER, D
    KEMP, DJ
    [J]. CELL, 1988, 53 (05) : 807 - 813