COMPARISON OF THE LIQUID-CHROMATOGRAPHIC BEHAVIOR OF SELECTED STEROID ISOMERS USING DIFFERENT REVERSED-PHASE MATERIALS AND MOBILE PHASE COMPOSITIONS

被引：18

作者：

OLSSON, M ^{[1
]}

SANDER, LC ^{[1
]}

WISE, SA ^{[1
]}

机构：

[1] NATL INST STAND & TECHNOL,CTR ANALYT CHEM,GAITHERSBURG,MD 20899

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 537卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88887-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The retention behaviour of steroid isomers in reversed-phase liquid chromatography was studied. Selectivity for the isomers changed as a function of both temperature and mobile-phase composition. In addition, the influence of the stationary phase on steroid retention and selectivity was studied for a number of monomeric and polymeric C-18 columns and beta-cyclodextrin columns. The selectivity of a polymeric C-18 phase toward various androstane standards was similar to that for a liquid crystal phase used in supercritical fluid chromatography.

引用

页码：73 / 83

页数：11

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