CLONING AND FUNCTIONAL EXPRESSION OF A HUMAN NEUROENDOCRINE VASOACTIVE-INTESTINAL-PEPTIDE RECEPTOR

被引：190

作者：

SREEDHARAN, SP ^{[1
]}

PATEL, DR ^{[1
]}

HUANG, JX ^{[1
]}

GOETZL, EJ ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL,SAN FRANCISCO,CA 94143

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.1658

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vasoactive intestinal peptide (VIP) is a potent mediator of gastrointestinal, nervous, pulmonary, vascular and immune functions. A cDNA was obtained from human HT29 intestinal epithelial cells and found to encode a 457 amino acid, 52 kDa VIP receptor. Transfection of the cDNA into COS-7 cells and 293 cells resulted in expression of specific saturable binding of VIP with a Kd of 0.8 nM, and induction of increases in intracellular cAMP by VIP with an EC50 of 1 nM. The human VIP receptor is homologous to other G protein-coupled receptors of the secretin-parathyroid hormone receptor family. A 2.8 kb transcript was detected in human lung, HT29 cells and Raji B-lymphoblasts with weaker expression in human brain, heart, kidney, liver and placenta. © 1993 Academic Press, Inc.

引用

页码：546 / 553

页数：8

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