MOSAIC EXPRESSION OF MEMBRANE PEPTIDASES BY CONFLUENT CULTURES OF CACO-2 CELLS

被引：24

作者：

HOWELL, S ^{[1
]}

BREWIS, IA ^{[1
]}

HOOPER, NM ^{[1
]}

KENNY, AJ ^{[1
]}

TURNER, AJ ^{[1
]}

机构：

[1] UNIV LEEDS,DEPT BIOCHEM & MOLEC BIOL,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND

来源：

FEBS LETTERS | 1993年 / 317卷 / 1-2期

基金：

英国惠康基金;

关键词：

MEMBRANE PEPTIDASE; IMMUNOFLUORESCENT STAINING; CACO-2; CELL;

D O I：

10.1016/0014-5793(93)81502-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The cell-surface expression of endopeptidase-24.11 (EC 3.4.24.11) on Caco-2 cells cultured to confluency is markedly heterogeneous unlike that of dipeptidylpeptidase IV (EC 3.4.14.5). Here we have investigated the cell-surface expression of three other ectopeptidases: angiotensin converting enzyme (EC 3.4.15.1), aminopeptidase N (EC 3.4.11.2) and aminopeptidase W (EC 3.4.11.16). We show by indirect immunofluorescent staining that these three enzymes are present on the surface of some cells but not on others. However, these enzymes were detected in the majority of detergent-permeabilised Caco-2 cells indicating the presence of intracellular pools of these enzymes. This suggests that there may either be differential regulation of apical transport for these peptidases or that they recycle at different rates.

引用

页码：109 / 112

页数：4

共 10 条

[1]

CHANTRET I, 1988, CANCER RES, V48, P1936

[2]

127 CULTURED HUMAN TUMOR-CELL LINES PRODUCING TUMORS IN NUDE MICE [J].