CRE-MEDIATED SITE-SPECIFIC TRANSLOCATION BETWEEN NONHOMOLOGOUS MOUSE CHROMOSOMES

被引：79

作者：

VANDEURSEN, J ^{[1
]}

FORNEROD, M ^{[1
]}

VANREES, B ^{[1
]}

GROSVELD, G ^{[1
]}

机构：

[1] ERASMUS UNIV ROTTERDAM, DEPT GENET, 3000 DR ROTTERDAM, NETHERLANDS

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 16期

关键词：

D O I：

10.1073/pnas.92.16.7376

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chromosome rearrangements, such as large deletions, inversions, of translocations, mediate migration of large DNA segments within or between chromosomes, which can have major effects on cellular genetic control. A method for chromosome manipulation would be very useful for studying the consequences of large-scale DNA rearrangements in mammalian cells or animals. With the use of the Cre-loxP recombination system of bacteriophage P1, we induced a site-specific translocation between the Dek gene on chromosome 13 and the Can gene on chromosome 2 in mouse embryonic stem cells. The estimated frequency of Cre-mediated translocation between the nonhomologous mouse chromosomes is approximately 1 in 1200-2400 embryonic stem cells expressing Cre recombinase. These results demonstrate the feasibility of site-specific recombination systems for chromosome manipulation in mammalian cells in vivo, breaking ground for chromosome engineering.

引用

页码：7376 / 7380

页数：5

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