LACTOSE-INHABITABLE AND HEPARIN-INHIBITABLE AGGLUTININ FROM HUMAN-FETAL BRAIN

被引：4

作者：

MANDAL, S ^{[1
]}

MAHAJAN, RG ^{[1
]}

机构：

[1] INDIAN INST CHEM BIOL,4 RAJA SC MULLICK RD,CALCUTTA 700032,W BENGAL,INDIA

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 01期

关键词：

Agglutinin characterization; Development; Human fetal brain;

D O I：

10.1111/j.1471-4159.1990.tb13313.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: An agglutinin activity which was sensitive to lactose and heparin was estimated during prenatal brain development. The agglutinin showed higher specific activities in cerebral cortex and midbrain. There was an increase in lectin specific activity in all the brain regions with development. In addition to brain, other fetal organs also showed the presence of developmentally regulated agglutinin. Cerebral cortical agglutinin was purified by Sepharose CL‐6B gel filtration and asialofetuin‐ and heparin‐Sepharose affinity chromatography. Purified agglutinin was strongly inhibited by lactose, asialofetuin, and heparin. It showed no requirement for divalent cations and was maximally active at pH 8.0. Electrophoretic characterization showed the aggregate nature of the agglutinin, and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis gave subunit molecular weights of 58,000, 45,000, and 24,000. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：288 / 293

页数：6

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