N-ACETYL-BETA-D-GLUCOSAMINIDASE ACTIVITY IN NORMAL AND CHRONIC LYMPHOCYTIC LEUKEMIC LYMPHOCYTES

被引:21
作者
CROCKARD, AD [1 ]
LEWIS, MHR [1 ]
BRIDGES, JM [1 ]
机构
[1] QUEENS UNIV BELFAST,DEPT BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND
关键词
D O I
10.1016/0009-8981(79)90256-0
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
N-Acetyl-β-d-glucosaminidase (EC 3.2.1.30) activity was measured fluori-metrically in: 1. (a) lymphocytes from 20 normal donors, 2. (b) enriched B and T lymphocyte populations prepared by E rosette sedi mentation from 8 normal subjects, 3. (c) lymphocytes from 15 untreated B cell chronic lymphocytic leukaemic patients. The pH profiles and optima (4.7) were similar in all preparations. Normal B lymphocytes had higher activity than normal T lymphocytes and both these preparations and normal unfractionated lymphocytes had significantly higher activity than chronic lymphocytic leukaemic lymphocytes. The apparent Michaelis constants were similar in normal unfractionated, B enriched and T enriched lymphocytes, whereas a reduced affinity for the enzyme was observed in the leukaemic lymphocytes. The difference in enzyme content between normal and chronic lymphocytic leukaemic lymphocytes cannot therefore be explained on the basis of a high B cell percentage in patients with chronic lymphocytic leukaemia. © 1979.
引用
收藏
页码:151 / 156
页数:6
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