THE STRUCTURE OF A PHASEOLUS-VULGARIS CDNA-ENCODING THE IRON STORAGE PROTEIN FERRITIN

被引：55

作者：

SPENCE, MJ

HENZL, MT

LAMMERS, PJ

机构：

[1] NEW MEXICO STATE UNIV,PROGRAM MOLEC BIOL,LAS CRUCES,NM 88003

[2] NEW MEXICO STATE UNIV,DEPT CHEM,LAS CRUCES,NM 88003

[3] NEW MEXICO STATE UNIV,PLANT GENET ENGN LAB,LAS CRUCES,NM 88003

来源：

PLANT MOLECULAR BIOLOGY | 1991年 / 17卷 / 03期

关键词：

5' UNTRANSLATED SEQUENCES; FRENCH BEAN; LAMBDA-GT11; PLANTS; PHYTOFERRITIN;

D O I：

10.1007/BF00040644

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda-gt11 cDNA library was constructed from seed-derived poly(A)+ RNA, and screened with the mouse anti-ferritin serum. A 1.2 kb immunopositive phage DNA insert was isolated and sequenced. The derived amino acid sequence shows substantial similarity with other ferritin sequences. The 5' untranslated region contains two out-of-frame AUG codons, a region of extreme pyrimidine composition bias and potentially stable secondary structure.

引用

页码：499 / 504

页数：6

共 28 条

[1] BOTH SUBUNITS OF RAT-LIVER FERRITIN ARE REGULATED AT A TRANSLATIONAL LEVEL BY IRON INDUCTION [J].