CELL IMMOBILIZATION USING PVA CROSS-LINKED WITH BORIC-ACID

被引：144

作者：

WU, KYA ^{[1
]}

WISECARVER, KD ^{[1
]}

机构：

[1] UNIV TULSA,DEPT CHEM ENGN,TULSA,OK 74104

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 04期

关键词：

IMMOBILIZED CELL; PVA; POLYVINYL ALCOHOL; FLUIDIZED BED;

D O I：

10.1002/bit.260390411

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new cell immobilization technique is described in which polyvinyl alcohol is crosslinked with boric acid, with the addition of a small amount of calcium alginate. The presence of the calcium alginate improves the surface properties of the beads, preventing agglomeration. A pure culture of phenol-degrading Pseudomonas was immobilized in the PVA-alginate beads. Phenol was successfully degraded in a fluidized bed of the beads, indicating that cell viability was maintained following the immobilization procedure. The PVA-alginate beads proved to be very strong and durable, with no noticeable degradation of the beads after 2 weeks of continuous operation of the fluidized bed.

引用

页码：447 / 449

页数：3

共 9 条

[1]

[Anonymous], 1985, STANDARD METHODS EXA

[2] IMMOBILIZATION OF MICROORGANISMS WITH PVA HARDENED BY ITERATIVE FREEZING AND THAWING [J].

ARIGA, O ;

TAKAGI, H ;

NISHIZAWA, H ;

SANO, Y .

JOURNAL OF FERMENTATION TECHNOLOGY, 1987, 65 (06) :651-658

[3]

GAUDY AF, 1982, EPA600282075

[4] IMMOBILIZATION OF ACTIVATED-SLUDGE BY PVA BORIC-ACID METHOD [J].

HASHIMOTO, S ;

FURUKAWA, K .

BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (01) :52-59

[5]

KINCANNON DF, 1983, J WATER POLLUT CON F, V55, P157

[6]

KUU WY, 1983, BIOTECHNOL BIOENG, V25, P1995, DOI 10.1002/bit.260250810

[7] COMPLEX-FORMATION BETWEEN POLYVINYL-ALCOHOL) AND BORATE ION [J].

OCHIAI, H ;

SHIMIZU, S ;

TADOKORO, Y ;

MURAKAMI, I .

POLYMER, 1981, 22 (11) :1456-1458

[8]

PETRASEK AC, 1983, J WATER POLLUT CON F, V55, P1286

[9]

Tampion J., 1987, IMMOBILIZED CELLS PR

← 1 →