HERPES-SIMPLEX VIRUS-INFECTION OF CULTURED HUMAN TERM TROPHOBLAST

被引：24

作者：

NORSKOVLAURITSEN, N ^{[1
]}

ABOAGYEMATHISEN, G ^{[1
]}

JUHL, CB ^{[1
]}

PETERSEN, PM ^{[1
]}

ZACHAR, V ^{[1
]}

EBBESEN, P ^{[1
]}

机构：

[1] DANISH CANC SOC,DEPT VIRUS & CANC,GUSTAV WIEDSVEJ 10,DK-8000 AARHUS C,DENMARK

来源：

JOURNAL OF MEDICAL VIROLOGY | 1992年 / 36卷 / 03期

关键词：

FETAL INFECTION; TRANSPLACENTAL; VIRUS-TRANSMISSION; VERTICAL; IMMUNOSORTING;

D O I：

10.1002/jmv.1890360303

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mononuclear trophoblast cells were isolated from term placentas of uncomplicated pregnancies, purified to homogeneity by negative immunomagnetic separation using monoclonal antibodies to the major histocompatibility complex, and challenged with herpes simplex virus (HSV). The cultures were highly susceptible to virus-induced cytopathic effect as evidenced by cytopathic alteration and inhibition of human chorionic gonadotropin (hCG) secretion. Both HSV I and II underwent a full replicative cycle in the trophoblast, although the production of progeny virus was 10-100 times less than that obtained with placental fibroblasts or choriocarcinoma cells. The permissiveness was independent of in vitro syncytial differentiation of the trophoblast. The results suggest that the trophoblast layer may be involved in intrauterine HSV infection.

引用

页码：162 / 166

页数：5

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