DESIGN PARAMETERS AND PERFORMANCE OF A SURFACE BAFFLED HELICAL RIBBON IMPELLER BIOREACTOR FOR THE CULTURE OF SHEAR SENSITIVE CELLS

被引：19

作者：

KAMEN, AA

CHAVARIE, C

ANDRE, G

ARCHAMBAULT, J

机构：

[1] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,OTTAWA K1A 0R6,ONTARIO,CANADA

[2] UNIV QUEBEC,TROIS RIVIERES GA9 5H7,QUEBEC,CANADA

来源：

CHEMICAL ENGINEERING SCIENCE | 1992年 / 47卷 / 9-11期

关键词：

D O I：

10.1016/0009-2509(92)87063-V

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

A novel bioreactor equipped with a double helical ribbon impeller (HRI) and three surface baffles has been shown to be well adapted to shear sensitive suspension cultures. Data on power dissipation, mixing time, circulation time, mass transfer parameters (k(L), k(L)a) were obtained at the 3 and 11 L scale. Rational design and scale-up of this type of unit are discussed. Performance of the HRI for high density cultivation of plant cells (27g dry weight/L) and insect cells (6xl0(6) cells/mL) are presented.

引用

页码：2375 / 2380

页数：6

共 26 条

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ARCHAMBAULT J, 1989, BIOTECHNOL BIOENG, V35, P702

[2]

CALDERBANK PH, 1961, T ICHEME, V39, P337

[3] HIGH-LEVEL RECOMBINANT PROTEIN-PRODUCTION IN BIOREACTORS USING THE BACULOVIRUS INSECT CELL EXPRESSION SYSTEM [J].