ELIMINATING NITRATE INTERFERENCE DURING KJELDAHL DIGESTION OF SOIL EXTRACTS FOR MICROBIAL BIOMASS DETERMINATION

被引：27

作者：

WYLAND, LJ ^{[1
]}

JACKSON, LE ^{[1
]}

BROOKS, PD ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY, DEPT SOIL SCI, BERKELEY, CA 94720 USA

来源：

SOIL SCIENCE SOCIETY OF AMERICA JOURNAL | 1994年 / 58卷 / 02期

关键词：

D O I：

10.2136/sssaj1994.03615995005800020016x

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Nitrate interference in Kjeldahl digestion of soil and plant samples presents a significant problem in establish N budgets in agricultural systems, where NO3- is frequently abundant. During digestion, NO3- can be reduced or it can cause volatilization of reduced N, resulting in either over- or under-estimation of Kjeldahl N. A method has been developed to volatilize NO3- from K2SO4 extracts of soil prior to Kjeldahl digestion for determination of microbial biomass N. Extracts were pretreated with H2O2 and H2SO4 at 97-degrees-C for 48 b, and then were Kjeldahl digested using a procedure modified for microbial biomass extracts. This method volatilizes >99% of NO3- in K2SO4 extracts, as determined by N-15 recovery from spiked samples.

引用

页码：357 / 360

页数：4

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