COMPARISON OF 2 HPLC TECHNIQUES FOR MONITORING ENANTIOSELECTIVE REACTIONS FOR THE RESOLUTION OF (R,S)-IBUPROFEN - CHIRAL HPLC VS ACHIRAL HPLC LINKED TO AN OPTICAL-ROTATION DETECTOR

Two approaches have been used to monitor the enantioselective esterification of (R,S)-ibuprofen with 1-dodecanol catalyzed by Novozym 435. The first approach involves the use of a chiral column (Chiralcel OD) connected to a UV detector. The second approach involves using a C-18 reversed-phase column connected in series to a RI detector and to an Optical Rotation detector; The two methods yield the same results.