IMMOBILIZATION OF ENZYMES ON TITANIUM-ACTIVATED INORGANIC SUPPORTS

Methods are described for the immobilization of enzymes on inorganic supports activated by the surface deposition of an imperfectly crystallized film of TiO//2. The stability of the preparations is shown to be strongly dependent on the integrity of the support-TiO//2 link which is in turn dependent on the pretreatment of the solid support. The enzyme may be linked to the activated support using 5-aminosalicylic acid (with or without diazotisation), tannic acid or stannous chloride, or by direct contact. These preparations are shown to have activities and operational stabilities superior to those of a preparation made using the silane-glutaraldehyde method on the same glass support.