VOLTAMMETRIC BEHAVIOR OF IMMUNOGLOBULIN-G AT STATIONARY MERCURY-ELECTRODES

被引：12

作者：

EMONS, H ^{[1
]}

WERNER, G ^{[1
]}

HEINEMAN, WR ^{[1
]}

机构：

[1] UNIV CINCINNATI,DEPT CHEM,CINCINNATI,OH 45221

来源：

ANALYST | 1990年 / 115卷 / 04期

关键词：

Adsorption; Alternating current voltammetry; Immunoglobulin G; Mercury electrode;

D O I：

10.1039/an9901500405

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A.c. voltammetric measurements have shown that bovine and human immunoglobulin G are adsorbed at a mercury electrode over a broad potential range and at open circuit. A current signal at ca. -0.55 V was identified as being due to an interfacial process connected with reversible protein re-orientations in the adsorption layer and, possibly, with fast faradaic reactions of both adsorbed redox states of the immunoglobulin.

引用

收藏

页码：405 / 408

页数：4

相关论文

共 21 条

[1] PATTERNS OF IONIC AND MOLECULAR ADSORPTION AT ELECTRODES [J].

ANSON, FC .

ACCOUNTS OF CHEMICAL RESEARCH, 1975, 8 (12) :400-406

[2]

BERG H, 1985, COMPR TREAT, V10, P189

[3] ADSORPTIVE STRIPPING VOLTAMMETRY OF F(AB')2 AND FAB FRAGMENTS OF ANTI-MOUSE IMMUNOGLOBULIN-G [J].

BUCKLEY, E ;

REILLY, P ;

FLORES, JR ;

OKENNEDY, R ;

SMYTH, MR .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1989, 7 (05) :543-547

[4]

DRYHURST G, 1982, BIOL ELECTROCHEMISTR, V1, P444

[5] STUDIES OF PROTEIN ADSORPTION ON POLYSTYRENE LATEX SURFACES [J].

FAIR, BD ;

JAMIESON, AM .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1980, 77 (02) :525-534

[6] THE APPLICATION OF ADSORPTIVE STRIPPING VOLTAMMETRY FOR A STUDY OF THE REACTION OF HUMAN-SERUM ALBUMIN WITH ANTI-HUMAN SERUM-ALBUMIN [J].

FLORES, JR ;

SMYTH, MR .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1987, 235 (1-2) :317-326

[7]

FONTAINE M, 1973, B SOC CHIM FR I-PHYS, P1873

[8] CYCLIC VOLTAMMETRY AND QUARTZ MICROBALANCE ELECTROGRAVIMETRY OF IGG AND ANTI-IGG REACTIONS ON SILVER [J].

GRABBE, ES ;

BUCK, RP ;

MELROY, OR .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1987, 223 (1-2) :67-78

[9]

GRANT WH, 1980, ADHESION ADSORPTION, P827

[10] ENZYMATIC ELECTROCATALYSIS AS A STRATEGY FOR ELECTROCHEMICAL DETECTION IN HETEROGENEOUS IMMUNOASSAYS [J].

GYSS, C ;

BOURDILLON, C .

ANALYTICAL CHEMISTRY, 1987, 59 (19) :2350-2355