MEASURING THE SURFACE-AREA OF A CELL BY THE METHOD OF THE SPATIAL GRID WITH A CSLM - A DEMONSTRATION

被引：27

作者：

HOWARD, CV ^{[1
]}

SANDAU, K ^{[1
]}

机构：

[1] UNIV HOHENHEIM,INST ANGEW MATH & STAT,W-7000 STUTTGART 70,GERMANY

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1992年 / 165卷

关键词：

STEREOLOGY; UNBIASED SURFACE ESTIMATION; OPTICAL SECTIONS; DESIGN-BASED SAMPLING; OSTEOCYTE LACUNAE; BONE HISTO-MORPHOMETRY;

D O I：

10.1111/j.1365-2818.1992.tb04315.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The spatial grid is a method for estimating the surface area of particles. A stack of perfectly registered sections is the essential prerequisite for its use. The confocal scanning light microscope provides such a stack by optical sectioning. The spatial grid method is briefly described and applied to an osteocyte lacuna in dry mineralized human mandible. This type of cell was chosen because of its very complex shape. The variance of the area estimate is studied and compared with the results of a simulation.

引用

页码：183 / 188

页数：6

共 7 条

[1] THE EFFICIENCY OF SYSTEMATIC-SAMPLING IN STEREOLOGY AND ITS PREDICTION
GUNDERSEN, HJG
JENSEN, EB
[J]. JOURNAL OF MICROSCOPY-OXFORD, 1987, 147 : 229 - 263
[2] Hahn U., 1989, ACTA STEREOL, V8, P425
[3] Inoue S, 1986, VIDEO MICROSCOPY
[4] ORTHOGONAL TRIPLET PROBES - AN EFFICIENT METHOD FOR UNBIASED ESTIMATION OF LENGTH AND SURFACE OF OBJECTS WITH UNKNOWN ORIENTATION IN SPACE
MATTFELDT, T
MOBIUS, HJ
MALL, G
[J]. JOURNAL OF MICROSCOPY-OXFORD, 1985, 139 (SEP): : 279 - 289
[5] AN INTERACTIVE IMAGE-ANALYSIS SYSTEM FOR MEAN PARTICLE-VOLUME ESTIMATION USING STEREOLOGICAL PRINCIPLES
MOSS, MC
BROWNE, MA
HOWARD, CV
JOYNER, DJ
[J]. JOURNAL OF MICROSCOPY-OXFORD, 1989, 156 : 79 - 90
[6] SANDAU K, 1987, ACTA STEREOL, V6, P31
[7] Santalo L. A., 2004, INTEGRAL GEOMETRY GE

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