IDENTIFICATION OF HUMAN AND BOVINE ROTAVIRUS SEROTYPES BY POLYMERASE CHAIN-REACTION

被引：111

作者：

TANIGUCHI, K

WAKASUGI, F

PONGSUWANNA, Y

URASAWA, T

UKAE, S

CHIBA, S

URASAWA, S

机构：

[1] SAPPORO MED COLL, DEPT PEDIAT, SAPPORO, HOKKAIDO 060, JAPAN

[2] NATL INST HLTH, VIRUS RES INST, NONTHABURI, THAILAND

来源：

EPIDEMIOLOGY AND INFECTION | 1992年 / 109卷 / 02期

关键词：

D O I：

10.1017/S0950268800050263

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The use of the polymerase chain reaction (PCR) for identifying serotypes of human and bovine rotaviruses was examined. In the identification of 115 human rotavirus samples in stools, results with PCR showed excellent agreement with results of serotyping by an enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies. Furthermore, the PCR showed a much higher sensitivity (93%) than the ELISA test (82.6%). The PCR method could also be applied for identifying the serotype of bovine rotaviruses.

引用

收藏

页码：303 / 312

页数：10

相关论文

共 34 条

[1] CHARACTERIZATION BY ENZYME-LINKED IMMUNOSORBENT-ASSAY USING SUBGROUP-SPECIFIC AND SEROTYPE-SPECIFIC MONOCLONAL-ANTIBODIES OF HUMAN ROTAVIRUS OBTAINED FROM DIARRHEIC PATIENTS IN BANGLADESH [J].

AHMED, MU ;

TANIGUCHI, K ;

KOBAYASHI, N ;

URASAWA, T ;

WAKASUGI, F ;

ISLAM, M ;

SHAIKH, H ;

URASAWA, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (07) :1678-1681

[2] TEMPORAL AND GEOGRAPHICAL DISTRIBUTIONS OF HUMAN ROTAVIRUS SEROTYPES, 1983 TO 1988 [J].

BEARDS, GM ;

DESSELBERGER, U ;

FLEWETT, TH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (12) :2827-2833

[3] PORCINE ROTAVIRUSES ANTIGENICALLY RELATED TO HUMAN ROTAVIRUS SEROTYPE-1 AND SEROTYPE-2 [J].

BELLINZONI, RB ;

MATTION, NM ;

MATSON, DO ;

BLACKHALL, J ;

LATORRE, JL ;

SCODELLER, EA ;

URASAWA, S ;

TANIGUCHI, K ;

ESTES, MK .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :633-636

[4] EPIDEMIOLOGY OF ROTAVIRUS SEROTYPES IN MELBOURNE, AUSTRALIA, FROM 1973 TO 1989 [J].

BISHOP, RF ;

UNICOMB, LE ;

BARNES, GL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (05) :862-868

[5] SEROLOGICAL AND GENOMIC CHARACTERIZATION OF L338, A NOVEL EQUINE GROUP-A ROTAVIRUS G-SEROTYPE [J].

BROWNING, GF ;

CHALMERS, RM ;

FITZGERALD, TA ;

SNODGRASS, DR .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :1059-1064

[6] A NOVEL GROUP-A ROTAVIRUS-G SEROTYPE - SEROLOGICAL AND GENOMIC CHARACTERIZATION OF EQUINE ISOLATE FI23 [J].

BROWNING, GF ;

FITZGERALD, TA ;

CHALMERS, RM ;

SNODGRASS, DR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (09) :2043-2046

[7] SIMPLE AND SPECIFIC ENZYME-IMMUNOASSAY USING MONOCLONAL-ANTIBODIES FOR SEROTYPING HUMAN ROTAVIRUSES [J].

COULSON, BS ;

UNICOMB, LE ;

PITSON, GA ;

BISHOP, RF .

JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (03) :509-515

[8] VARIATION IN NEUTRALIZATION EPITOPES OF HUMAN ROTAVIRUSES IN RELATION TO GENOMIC RNA POLYMORPHISM [J].

COULSON, BS .

VIROLOGY, 1987, 159 (02) :209-216

[9]

EISENSTEIN BI, 1990, NEW ENGL J MED, V322, P178

[10] ROTAVIRUS GENE STRUCTURE AND FUNCTION [J].

ESTES, MK ;

COHEN, J .

MICROBIOLOGICAL REVIEWS, 1989, 53 (04) :410-449

← 1 2 3 4 →