MOLECULAR-CLONING OF THE ANTIBACTERIAL PROTEIN OF THE GIANT AFRICAN SNAIL, ACHATINA-FULICA FERUSSAC

被引:41
作者
OBARA, K
OTSUKAFUCHINO, H
SATTAYASAI, N
NONOMURA, Y
TSUCHIYA, T
TAMIYA, T
机构
[1] SOPHIA UNIV,FAC SCI & TECHNOL,DEPT CHEM,7-1 KIOI CHO,CHIYODA KU,TOKYO 102,JAPAN
[2] KHON KAEN UNIV,FAC SCI & TECHNOL,DEPT CHEM,KHON KAEN,THAILAND
[3] UNIV TOKYO,FAC MED,DEPT PHARMACOL,TOKYO 113,JAPAN
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 209卷 / 01期
关键词
D O I
10.1111/j.1432-1033.1992.tb17254.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An expression cDNA library was constructed with poly(A)-rich RNA extracted from the collar of the giant African snail, Achatina fulica Ferussac. A 1.9-kbp cDNA clone encoding a precursor of antibacterial glycoprotein of the snail, achacin, was isolated from the cDNA expression library. The cDNA sequence contains an open reading frame with 1593-nucleotide residues. The deduced amino acid sequence of this achacin precursor starts with a 29-residue leader peptide followed by a 502-residue mature peptide (56 kDa) with four possible N-glycosylation sites, Asn-Xaa-Ser or Asn-Xaa-Thr. The Northern-blot analysis proved that the achacin precursor was specifically expressed in the tissue of snail collar and processed to mature achacin. cDNA inserts encoding achacin precursor were subcloned into expression plasmids. Three kinds of expressed polypeptides were cross-reacted with rabbit antiserum raised against achacin. The largest polypeptide (M(r) 63000) should be the achacin precursor.
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页码:1 / 6
页数:6
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