CHARGE-TRANSFER AND WATER-MEDIATED CHROMATOGRAPHY .2. ADSORPTION OF NUCLEOTIDES AND RELATED COMPOUNDS ON ACRIFLAVIN-SEPHADEX

Low-molecular-weight compounds are linearly adsorbed on acriflavin-Sephadex, and desorption by variation of pH, salt or temperature is therefore not necessary. The VE/VT values of the tested substances under the experimental conditions varied in the range 1-15, thus allowing good separations on moderate-sized columns. The adsorption capacity is surprisingly high, indicating the great potentiality for large-scale fractionation of oligonucleotides. Secondary cooperative adsorption effects can be controlled by temperature, pH, ionic strength and/or polarity of the eluent (which should increase the versatility of charge-transfer adsorption in nucleotide chemistry). © 1979.