COUPLING OF ENZYMES TO PROTEINS WITH GLUTARALDEHYDE . USE OF CONJUGATES FOR DETECTION OF ANTIGENS AND ANTIBODIES

被引：1667

作者：

AVRAMEAS, S

机构：

[1] Laboratoire de Chimie des Protéines, Institut de Recherches Scientifiques sur le Cancer, Villejuif

来源：

IMMUNOCHEMISTRY | 1969年 / 6卷 / 01期

关键词：

D O I：

10.1016/0019-2791(69)90177-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conjugation of peroxidase, glucose oxidase, tyrosinase and alkaline phosphatase to human immunoglobulin-G, human serum albumin, sheep antibody and rabbit antibody was carried out with glutaraldehyde. The conjugates retained a substantial part of their immunological and enzymatic activity. They were utilized for the intracellular detection of antigens and antibodies and they were employed for the characterization of the antibodies after immunoelectrophoresis. © 1969.

引用

页码：43 / +

页数：1

共 25 条

[1]

AVRAMEAS S, 1967, CR ACAD SCI D NAT, V265, P1149

[2]

AVRAMEAS S, 1966, CR ACAD SCI D NAT, V262, P2543

[3] CROSS-LINKING OF PROTEINS WITH GLUTARALDEHYDE AND ITS USE FOR PREPARATION OF IMMUNOADSORBENTS [J].