EFFECTS OF INVITRO EXPOSURE TO HCG ON SUBSEQUENT HCG-RESPONSIVENESS OF HUMAN GRANULOSA-CELLS OBTAINED FOLLOWING TREATMENT WITH GNRH ANALOG AND GONADOTROPINS - AN INVITRO MODEL FOR LUTEAL PHASE SUPPORT

被引：7

作者：

EDGAR, DH

WHALLEY, KM

GEMMELL, JA

JAMES, GB

MILLS, JA

机构：

[1] Unit of Reproductive Medicine, Ninewells Hospital, Medical School

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 02期

关键词：

GNRH ANALOG; GRANULOSA CELLS INVITRO; HCG; LUTEAL PHASE SUPPORT;

D O I：

10.1093/oxfordjournals.humrep.a137305

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The pregnancy rate in patients undergoing assisted conception treatment following pituitary desensitization with GnRH analogue and ovarian stimulation with gonadotrophins has been reported to be higher when ovarian function is supported in the luteal phase by exogenous human chorionic gonadotrophin (HCG). In the present study, we have examined the effects of culturing monolayers of granulosa cells, collected from such patients at oocyte retrieval, for various time intervals in the presence or absence of HCG on their subsequent ability to secrete progesterone (P4) either spontaneously or in response to further challenge with HCG. When cultured in the absence of HCG, granulosa cells demonstrated a rapid decline in both the spontaneous P4 secretion rate and the ability to secrete P4 spontaneously and also significantly enhanced the ability to respond to subsequent HCG stimulation. These results suggest that HCG support in the luteal phase in GnRH analogue-treated patients may have a cellular basis for its action both in maintenance of P4 secretion and also in rendering the corpus luteum more sensitive to rescue by conceptus-derived HCG.

引用

页码：198 / 202

页数：5

共 17 条

[1]

Baird D.T., Control of luteolysis, The Luteal Phase, pp. 25-42, (1985)

[2]

Bar-Ami S., Haciski R.C., Channing C.P., Increasing [125I]human chorionic gonadotrophin specific binding in human granulosa cells by follicle-stimulating hormone and follicular fluid, Hum. Reprod, 4, pp. 876-882, (1989)

[3]

Casper R.F., Erickson G.F., Yen S.S.C., Studies on the effect of gonadotropin-releasing hormone and its agonist on human luteal steroidogenesis in vitro, Fertil. Steril, 42, pp. 39-43, (1984)

[4]

Corker C.S., Michie E., Hobson B., Parboosingh J., Hormonal patterns in conceptual cycles and early pregnancy, Br. J. Obstet. Gynaecol, 83, pp. 489-494, (1976)

[5]

Edwards R.G., Steptoe P.C., Fowler R.E., Baillie J., Observations on preovulatory human ovarian follicles and their aspirates, Br. J. Obstet. Gynaecol, 87, pp. 769-779, (1980)

[6]

Fisch B., Margara R.A., Winston R.M.L., Hillier S.G., Cellular basis of luteal steroidogenesis in the human ovary, J. Endocrinol, 122, pp. 303-311, (1989)

[7]

Fleming R., Coutts J.R.T., Induction of multiple follicular growth in normally menstruating women with endogenous gonadotropin suppression, Fertil. Steril, 45, pp. 226-230, (1986)

[8]

Hillier S.G., Wickings E.J., Afnan M., Margara R.A., Winston R.M.L., Granulosa cell steroidogenesis before in vitro fertilization, Biol. Reprod, 31, pp. 679-686, (1984)

[9]

Lanzone A., Panetta V., Disimone N., Arno E., Fulghesu A.M., Caruso A., Mancuso S., Effect of gonadotrophin-releasing hormone and related analogue on human luteal cell function in vitro, Hum. Reprod, 4, pp. 906-909, (1989)

[10]

McNatty K.P., Makris A., Degrazia C., Osathanondh R., Ryan K.J., The production of progesterone, androgens, and estrogens by granulosa cells, thecal tissue, and stromal tissue from human ovaries in vitro, J. Clin. Endocrinol. Metab, 49, pp. 687-699, (1979)

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