DIFFERENT TRANSLOCATION OF 3 DISTINCT PKC ISOFORMS WITH TUMOR-PROMOTING PHORBOL ESTER IN HUMAN PLATELETS

被引：86

作者：

CRABOS, M ^{[1
]}

IMBER, R ^{[1
]}

WOODTLI, T ^{[1
]}

FABBRO, D ^{[1
]}

ERNE, P ^{[1
]}

机构：

[1] UNIV HOSP BASEL,DEPT RES,CH-4031 BASEL,SWITZERLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90973-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein kinase C (PKC), a family of related but distinct enzymes whose cellular functions are poorly understood, acts in synergy with Ca2+ mobilization for the activation of platelets. Using specific antibodies for the different isoforms, immunoblot analysis revealed the presence in human platelets of three different PKC subtypes which specifically react with α, β and ζ-PKC antibodies. Whereas the subcellular distribution of the α PKC remained unaffected, incubation of platelets with 1 μM PMA for 2 min resulted in a significant subcellular distribution from cytosol to membrane of β-PKC (25%) and ζ (15%). The β-PKC isoform is more sensitive than α and ζ-PKC to PMA, since 100 nM PMA resulted in a translocation of 85%, 64% and 66% respectively of a maximum translocation observed with 1 μM PMA. © 1991.

引用

页码：878 / 883

页数：6

共 26 条

[1] ISOLATION AND CHARACTERIZATION OF PKC-L, A NEW MEMBER OF THE PROTEIN-KINASE C-RELATED GENE FAMILY SPECIFICALLY EXPRESSED IN LUNG, SKIN, AND HEART [J].