GENOMIC CHARACTERIZATION OF PHENOTYPIC VARIANTS OF BEET CURLY TOP VIRUS

被引：59

作者：

STENGER, DC ^{[1
]}

CARBONARO, D ^{[1
]}

DUFFUS, JE ^{[1
]}

机构：

[1] USDA ARS,SALINAS,CA 93905

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-10-2211

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Full-length infectious DNA clones were constructed for four distinct phenotypic variants of beet curly top virus (BCTV). Southern hybridization assays indicated that each cloned BCTV genome shared sequence homology with pBCT-028, a full-length infectious DNA clone of a California isolate of BCTV previously characterized by others. Restriction endonuclease maps of the cloned BCTV genomes were distinct from one another. Infectivity assays determined that plasmids containing tandem repeats of BCTV genomes were generally more infectious than excised linear DNA inserts. Progeny virus, derived from plants inoculated with clones DNAs, differed in their ability to infect sugarbeet, Beta vulgaris L., and the severity of symptoms produced in B. vulgaris and other experimental hosts.

引用

页码：2211 / 2215

页数：5

共 18 条

[1]

BENNETT CW, 1971, MONOGRAPHS AM PHYTOP, V7, P26

[2] THE COAT PROTEIN OF BEET CURLY TOP VIRUS IS ESSENTIAL FOR INFECTIVITY [J].