DIBUTYRYL CAMP ACTIVATES BUMETANIDE-SENSITIVE ELECTROLYTE TRANSPORT IN MALPIGHIAN TUBULES

被引:89
作者
HEGARTY, JL [1 ]
ZHANG, B [1 ]
PANNABECKER, TL [1 ]
PETZEL, DH [1 ]
BAUSTIAN, MD [1 ]
BEYENBACH, KW [1 ]
机构
[1] CORNELL UNIV,DIV BIOL SCI,PHYSIOL SECT,VRT 826,ITHACA,NY 14853
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 261卷 / 03期
关键词
AEDES-AEGYPTI; TRANSEPITHELIAL SODIUM; POTASSIUM; AND CHLORIDE SECRETION; ELECTRON PROBE ANALYSIS; SECRETORY DIURESIS; TRANSEPITHELIAL VOLTAGE AND RESISTANCE; MEMBRANE VOLTAGE AND FRACTIONAL RESISTANCE; OUABAIN; 4-ACETAMIDO-4'-ISOTHIOCYNANOSTILBENE-2,2'-DISULFONIC ACID; DIBUTYRYL ADENOSINE 3'; 5'-CYCLIC MONOPHOSPHATE;
D O I
10.1152/ajpcell.1991.261.3.C521
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The effects of dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) and bumetanide (both 10(-4) M) on transepithelial Na+, K+, Cl-, and fluid secretion and on tubule electrophysiology were studied in isolated Malpighian tubules of the yellow fever mosquito Aedes aegypti. Peritubular DBcAMP significantly increased Na+, Cl-, and fluid secretion but decreased K+ secretion. In DBcAMP-stimulated tubules, bumetanide caused Na+, Cl-, and fluid secretion to return to pre-cAMP control rates and K+ secretion to decrease further. Peritubular bumetanide significantly increased Na+ secretion and decreased K+ secretion so that Cl- and fluid secretion did not change. In bumetanide-treated tubules, the secretagogue effects of DBcAMP are blocked. In isolated Malpighian tubules perfused with symmetrical Ringer solution, DBcAMP significantly hyperpolarized the transepithelial voltage (V(T)) and depolarized the basolateral membrane voltage (V(bl)) with no effect on apical membrane voltage (V(a)). Total transepithelial resistance (R(T)) and the fractional resistance of the basolateral membrane (fR(bl)) significantly decreased. Bumetanide also hyperpolarized V(T) and depolarized V(bl), however without significantly affecting R(T) and fR(bl). Together these results suggest that, in addition to stimulating electroconductive transport, DBcAMP also activates a nonconductive bumetanide-sensitive transport system in Aedes Malpighian tubules.
引用
收藏
页码:C521 / C529
页数:9
相关论文
共 33 条
[1]   OUABAIN-SENSITIVITY OF INSECT EPITHELIAL TISSUES [J].
ANSTEE, JH ;
BOWLER, K .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 1979, 62 (04) :763-769
[2]  
BALDRICK P, 1988, J INSECT PHYSL, V10, P963
[3]   ELECTROPHYSIOLOGICAL EVIDENCE FOR CL SECRETION IN SHARK RENAL PROXIMAL TUBULES [J].
BEYENBACH, KW ;
FROMTER, E .
AMERICAN JOURNAL OF PHYSIOLOGY, 1985, 248 (02) :F282-F295
[4]  
BEYENBACH KW, 1987, NEWS PHYSIOL SCI, V2, P171
[5]   PREPARATION AND STUDY OF FRAGMENTS OF SINGLE RABBIT NEPHRONS [J].
BURG, M ;
GRANTHAM, J ;
ABRAMOW, M ;
ORLOFF, J .
AMERICAN JOURNAL OF PHYSIOLOGY, 1966, 210 (06) :1293-&
[6]   ANISOSMOTIC CELL-VOLUME REGULATION - A COMPARATIVE VIEW [J].
CHAMBERLIN, ME ;
STRANGE, K .
AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (02) :C159-C173
[7]   NA-K-CL COTRANSPORT IN CHLORIDE-TRANSPORTING EPITHELIA [J].
EPSTEIN, FH ;
SILVA, P .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1985, 456 :187-197
[8]   EFFECTS OF EPINEPHRINE, GLUCAGON AND VASOACTIVE INTESTINAL POLYPEPTIDE ON CHLORIDE SECRETION BY TELEOST OPERCULAR MEMBRANE [J].
FOSKETT, JK ;
HUBBARD, GM ;
MACHEN, TE ;
BERN, HA .
JOURNAL OF COMPARATIVE PHYSIOLOGY, 1982, 146 (01) :27-34
[9]   SODIUM-COUPLED CHLORIDE TRANSPORT BY EPITHELIAL TISSUES [J].
FRIZZELL, RA ;
FIELD, AM ;
SCHULTZ, SG .
AMERICAN JOURNAL OF PHYSIOLOGY, 1979, 236 (01) :F1-F8