A XERODERMA-PIGMENTOSUM COMPLEMENTATION GROUP-A RELATED GENE - CONFIRMATION USING MONOCLONAL-ANTIBODIES AGAINST THE CYCLOBUTANE DIMER AND (6-4) PHOTOPRODUCT

被引：13

作者：

MORI, T

RINALDY, TL

ATHWAL, RS

KAUR, GP

NIKAIDO, O

LLOYD, RS

RINALDY, A

机构：

[1] UNIV TENNESSEE CTR HLTH SCI,DEPT MED,956 COURT AVE,ROOM H-300,MEMPHIS,TN 38163

[2] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT MICROBIOL & MOLEC GENET,NEWARK,NJ 07103

[3] KANAZAWA UNIV,FAC PHARMACEUT SCI,DIV RADIAT BIOL,KANAZAWA,ISHIKAWA 920,JAPAN

[4] VANDERBILT UNIV,DEPT BIOCHEM,NASHVILLE,TN 37232

[5] CTR MOLEC TOXICOL,NASHVILLE,TN 37232

[6] NARA MED UNIV,RI CTR,KASHIHARA,NARA 634,JAPAN

[7] VET AFFAIRS MED CTR,MEMPHIS,TN 38104

来源：

MUTATION RESEARCH | 1993年 / 293卷 / 02期

关键词：

XERODERMA-PIGMENTOSUM; DNA-REPAIR GENE; CYCLOBUTANE DIMER; (6-4) PHOTOPRODUCT; COMPLEMENTATION GROUP-A;

D O I：

10.1016/0921-8777(93)90065-O

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Xeroderma pigmentosum complementation group A was partially complemented by a cosmid genomic clone containing a 42-kb human DNA insert selected with a cDNA clone that we obtained through cDNA competition between the repair-proficient and repair-deficient cell line. The relationship between these two clones was confirmed using PCR amplifications. The enhancement in DNA-repair capacity of the transformants was assessed with the monoclonal antibodies specific for cyclobutane dimers and (6-4) photoproducts and partially correct the xeroderma pigmentosum complementation group A defect. Furthermore, the level of the photoproduct-repair capacity is in agreement with the survival enhancement calculated from the D37 values. This gene was mapped to chromosome 8, suggesting that this may represent one of the defective gene(s) in xeroderma pigmentosum complementation group A.

引用

页码：143 / 150

页数：8

共 40 条

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