DEVELOPMENTAL GENE-EXPRESSION OF A 230-KILODALTON MACROGAMETE-SPECIFIC PROTEIN OF THE AVIAN COCCIDIAL PARASITE, EIMERIA-MAXIMA

被引：31

作者：

FRIED, M ^{[1
]}

MENCHER, D ^{[1
]}

SARSHALOM, O ^{[1
]}

WALLACH, M ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,KUVIN CTR STUDY INFECT & TROP DIS,POB 1172,IL-91010 JERUSALEM,ISRAEL

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1992年 / 51卷 / 02期

关键词：

DNA SEQUENCING; INSITU HYBRIDIZATION; WALL-FORMING BODIES;

D O I：

10.1016/0166-6851(92)90075-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We prepared a cDNA library from gametocytes of Eimeria maxima and screened it using antibodies raised against an 82-kDa gametocyte antigen. One cDNA clone designated pEM230 was isolated and characterized. It encodes a portion of a 230-kDa gametocyte protein and its DNA sequence shows the presence of several tandem repeats of 42 bp. In order to determine the stage and sex specificity of the mRNA for the 230-kDa protein, Northern blotting and in situ hybridization studies were performed. The 230-kDa protein is encoded for by a 7 kb mRNA, which is expressed exclusively during the macrogamete stage with no detectable expression seen in any other stage of parasite development.

引用

页码：251 / 262

页数：12

共 15 条

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