AN HSP70 HOMOLOG IS ENCODED ON THE PLASTID GENOME OF THE RED ALGA, PORPHYRA-UMBILICALIS

被引：31

作者：

REITH, M

MUNHOLLAND, J

机构：

[1] Institute for Marine Biosciences, National Research Council of Canada, Halifax, NS B3H 3Z1

来源：

FEBS LETTERS | 1991年 / 294卷 / 1-2期

关键词：

DNAK; HEAT SHOCK; HSP70; PLASTID GENOME; RED ALGA; TRNG(GCC);

D O I：

10.1016/0014-5793(91)81355-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A PCR experiment using Porphyra umbilicalis DNA as the template and degenerate oligonucleotides representing conserved regions of hsp70 amino acid sequences generated a 1 kb product that hybridized exclusively to the plastid DNA of this red alga. DNA sequencing of two contiguous EcoRI plastid DNA clones revealed a 620 amino acid open reading frame with 71 % identity to the dnaK gene of the cyanobacterium, Synechocystis 6803. Northern hybridization experiments detected a 2.3 kb transcript that is present in control (15-degrees-C) cultures and increases approximately 7-fold upon heat shock (75 minutes at 30-degrees-C).

引用

页码：116 / 120

页数：5

共 28 条

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