EXPRESSION OF THE BGP GENE AND CHARACTERIZATION OF MOUSE COLON BILIARY GLYCOPROTEIN ISOFORMS

被引:62
作者
MCCUAIG, K
ROSENBERG, M
NEDELLEC, P
TURBIDE, C
BEAUCHEMIN, N
机构
[1] MCGILL UNIV,MCGILL CANC CTR,3655 DURMMOND ST,MONTREAL H3G 1Y6,QUEBEC,CANADA
[2] MCGILL UNIV,DEPT BIOCHEM,MONTREAL H3A 2T5,QUEBEC,CANADA
[3] MCGILL UNIV,DEPT MED,MONTREAL H3A 2T5,QUEBEC,CANADA
关键词
CARCINOEMBRYONIC ANTIGEN GENE FAMILY MEMBER; REVERSE TRANSCRIPTION AND POLYMERASE CHAIN AMPLIFICATION; MOUSE HEPATITIS VIRUS RECEPTOR; ADHESION MOLECULE;
D O I
10.1016/0378-1119(93)90716-G
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The biliary glycoprotein (BGP)-encoding gene is a member of the human carcinoembryonic antigen (CEA) gene family. We have now cloned several mouse Bgp cDNAs from an outbred CD(R)-1 mouse colon cDNA library, as well as by reverse transcription-PCR amplification of colon RNA. The distinguishing features of the deduced Bgp protein isoforms are found in the two divergent N-terminal domains, the highly conserved internal C2-set immunoglobulin domains, and an intracytoplasmic domain of either 10 or 73 amino acids (aa). The cDNA structures suggest that these mRNAs are produced through alternative splicing of a Bgp gene and the usage of multiple transcriptional terminators. The Bgp deduced aa sequences are highly homologous to several well characterized rat hepatocyte proteins such as the cell CAM105/ecto-ATPase/pp120/HA4 proteins. Oligodeoxyribonucleotide probes representing the various cDNA isoform domains revealed predominant transcripts of 1.8, 3.1 and 4.0 kb on Northern analyses of mouse colon RNA; some of these bands are actually composed of several co-migrating transcripts. The transcripts encoding the long intracytoplasmic-tailed Bgp proteins are expressed at one-tenth the relative abundance of the shorter-tailed species. We have previously demonstrated that several mouse Bgp cDNAs, when transfected into eukaryotic cells, express BGP proteins at the cell surface and function in vitro as cell adhesion molecules, much like their human and rat counterparts. The expression of the many Bgp isoforms at the surface of epithelial cells, such as colon. suggests that these proteins play a determinant role, through self- or heterologous contact, in renewal and/or differentiation of their epithelia.
引用
收藏
页码:173 / 183
页数:11
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