OPTICAL METHOD, FOR MEASUREMENT OF NUMBER AND SHAPE OF ATTACHED CELLS IN REAL-TIME

被引：50

作者：

RAMSDEN, JJ ^{[1
]}

LI, SY ^{[1
]}

HEINZLE, E ^{[1
]}

PRENOSIL, JE ^{[1
]}

机构：

[1] ETH ZURICH,DEPT CHEM ENGN,ZURICH,SWITZERLAND

来源：

CYTOMETRY | 1995年 / 19卷 / 02期

关键词：

BHK CELLS; ATTACHMENT KINETICS; SPREADING KINETICS; INTEGRATED OPTICS; PLANAR WAVE-GUIDE; GRATING COUPLER;

D O I：

10.1002/cyto.990190202

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel, on-line method of accurately parametrizing the shape of spreading cells is described. The substrate on which the cells are deposited serves as an optical waveguide. The method is based on the interaction between the evanescent part of the guided waves and the cells. No special labelling of the cells is required. A mathematical framework for interpreting the results is derived, and some illustrative results are presented. (C) 1995 Wiley-Liss, Inc.

引用

页码：97 / 102

页数：6

共 19 条

[1]

Alberts B., 1989, MOL BIOL CELL

[2] PHYSICS OF CELL-ADHESION [J].