IDENTIFICATION OF A CYTOLYTIC PROTEIN IN THE CELOMIC FLUID OF EISENIA-FOETIDA EARTHWORMS

被引:73
作者
BILEJ, M
BRYS, L
BESCHIN, A
LUCAS, R
VERCAUTEREN, E
HANUSOVA, R
DEBAETSELIER, P
机构
[1] FREE UNIV BRUSSELS,INST MOLEC BIOL,CELLULAR IMMUNOL UNIT,B-1640 RHODE ST GENESE,BELGIUM
[2] CHARLES UNIV,FAC NAT SCI,CR-12000 PRAGUE,CZECH REPUBLIC
关键词
EISENIA FOETIDA; CELOMIC FLUID; CYTOLYTIC ACTIVITY; TUMOR CELL LINE; HEMOLYSIS;
D O I
10.1016/0165-2478(94)00248-P
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Total coelomic fluid of earthworms Eisenia foetida (Oligochaeta, Annelida) is capable of lysing different mammalian tumor cell lines. This cytolytic activity is different from tumor necrosis factor (TNF)-mediated lysis and is not due to proteolysis. Total coelomic fluid was subjected to ion-exchange chromatography separation and a fraction with prominent cytolytic activity was used to elicit monoclonal antibodies that were screened for their capacity to neutralize the cytolytic effect of total coelomic fluid. One of the prepared neutralizing IgG antibodies was used for the immunoaffinity purification of a cytolytic factor from total coelomic fluid. SDS-PAGE and Western blot analyses revealed a protein band with an apparent molecular weight of 42 kDa. This cytolytic protein (termed CCF-1 or coelomic cytolytic factor 1) can be adsorbed on the surface of opsonized particles and may be involved in opsonizing and hemolytic effects of coelomic fluid.
引用
收藏
页码:123 / 128
页数:6
相关论文
共 15 条
[1]   PRIMITIVE CYTOKINES - HARBINGERS OF VERTEBRATE DEFENSE [J].
BECK, G ;
HABICHT, GS .
IMMUNOLOGY TODAY, 1991, 12 (06) :180-183
[2]   THE FLOW CYTOMETRIC ANALYSIS OF INVITRO PHAGOCYTIC-ACTIVITY OF EARTHWORM CELOMOCYTES (EISENIA-FOETIDA, ANNELIDA) [J].
BILEJ, M ;
SCHEERLINCK, JP ;
VANDENDRIESSCHE, T ;
DEBAETSELIER, P ;
VETVICKA, V .
CELL BIOLOGY INTERNATIONAL REPORTS, 1990, 14 (09) :831-837
[3]  
BILEJ M, 1990, FOLIA BIOL-PRAGUE, V36, P273
[4]  
Bilej Martin, 1994, P245
[5]  
FORNUSEK L, 1986, Critical Reviews in Therapeutic Drug Carrier Systems, V2, P137
[6]   A NEW SOURCE OF BIOLOGICALLY-ACTIVE COMPOUNDS EARTHWORM TISSUE (EISENIA-FOETIDA, LUMBRICUS-RUBELUS) [J].
HRZENJAK, T ;
HRZENJAK, M ;
KASUBA, V ;
EFENBERGERMARINCULIC, P ;
LEVANAT, S .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 1992, 102 (03) :441-447
[7]   CYTOTOXIC ACTIVITY IN THE CELOMIC FLUID OF THE ANNELID EISENIA-FOETIDA SAV [J].
KAUSCHKE, E ;
MOHRIG, W .
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY, 1987, 157 (01) :77-83
[8]  
LUCAS R, 1990, IMMUNOLOGY, V71, P218
[9]  
NAGASAWA H, 1991, ANTICANCER RES, V11, P1061
[10]   THE PROTEINS IN LUMBRICUS-TERRESTRIS AND EISENIA FOETIDA CELOMIC FLUIDS AND ON CELOMOCYTES REACTING WITH SHEEP AND GOAT IGG MOLECULES [J].
REJNEK, J ;
TUCKOVA, L ;
SIMA, P ;
KOSTKA, J .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1986, 10 (04) :467-475